Journal: Biofabrication

Article Title: Aqueous two-phase deposition and fibrinolysis of fibroblast-laden fibrin micro-scaffolds

doi: 10.1088/1758-5090/abdb85

Figure Lengend Snippet: Cell donor and drug stimulation: Time-course pixel intensity data show the effects on fibrin degradation of several different stimulants, with NHLF cells on the left and diseased IPF cells on the right. The upper pixel intensity graphs have no TGF-β1 (a and e) while the lower graphs contain 2 ng/ml TGF-β1 (b and f). Sigmoid fits were used to determine 50% degradation time (c, d, g and h) from the above pixel intensity graphs. Dotted lines show mean value from control conditions for comparison. (Statistical significance P < 0.01 by two-way ANOVA: As the positive control, plasmin was excluded from ANOVA. ‡ = P < 0.01; ad, be = P < 0.05; ac, fg, fh = P < 0.1 by post-hoc Tukey test. N = 4 for all conditions)

Article Snippet: Cell preparation Normal human lung fibroblasts (NHLF lot#0000580583; Lonza) from a 79 year old female with a history of smoking, and idiopathic pulmonary fibrosis fibroblasts (IPF lot#0000627840; Lonza) from a 52 year old male were cultured in fibroblast growth media (FGM; Lonza).

Techniques: Control, Comparison, Positive Control

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Microvesicles Derived From Human Mesenchymal Stem Cells Restore Alveolar Fluid Clearance in Human Lungs Rejected for Transplantation

doi: 10.1111/ajt.13271

Figure Lengend Snippet: (A) Schematic view of the ex vivo human lung preparation as well as lung perfusion and ventilation protocol used for the experiments. (B) Electron microscopy image of human mesenchymal stem cells (MSC) constitutively releasing microvesicles (MV) after 48 h of serum starvation. Black arrows designate MV, which appear as spheroids budding off the plasma membrane. Insert shows a homogenous collection of MSC MV, which is 50 to 200 nm in size. (C) Protein concentration of 100 μL of MSC MV. Data represent the median of 24 samples of MSC MV. (D) Western Blot showing the expression of the membrane receptor CD44 by MSC MV. CD44 plays an important role in MSC MV internalization into the host cell. PCR showing the expression of Ang1 mRNA by RT-PCR.

Article Snippet: If 0 microvesicles derived from normal human lung fibroblasts (NHLF MV, Lonza, Basel, Switzerland) was administered into the perfusate.

Techniques: Ex Vivo, Electron Microscopy, Protein Concentration, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Microvesicles Derived From Human Mesenchymal Stem Cells Restore Alveolar Fluid Clearance in Human Lungs Rejected for Transplantation

doi: 10.1111/ajt.13271

Figure Lengend Snippet: Administration of MSC MV significantly improved AFC in a dose-dependent manner compared to the Control (Perfusion only) at T6. Administration of NHLF MV as a cellular control had no effect on AFC compared to the Control at T6. Inhibition of CD44 with a neutralizing Ab significantly reduced the therapeutic effect of MSC MV 200 μL, whereas administration of IgG control with MSC MV 200 μL had no significant effect. Data are presented as mean ± SD, N = 4-6 per group. *p significant by ANOVA vs. T0, †p significant by ANOVA versus T6 Perfusion Only, ‡p significant by ANOVA versus T6 MSC MV 200 μL. Abbreviations: Ab, antibody; AFC, alveolar fluid clearance; MSC, mesenchymal stem cells; MV, microvesicles; NHLF, normal human lung fibroblast.

Article Snippet: If 0 microvesicles derived from normal human lung fibroblasts (NHLF MV, Lonza, Basel, Switzerland) was administered into the perfusate.

Techniques: Inhibition

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Microvesicles Derived From Human Mesenchymal Stem Cells Restore Alveolar Fluid Clearance in Human Lungs Rejected for Transplantation

doi: 10.1111/ajt.13271

Figure Lengend Snippet: Effect of microvesicles derived from human mesenchymal stem cells on lung (A) tracheal pressure and (B) compliance. Perfusion with ventilation significantly increased tracheal pressure at T6. Administration of either doses of MSC MV reduced the tracheal pressure to baseline, increasing the compliance of the lung compared to T0. Data are presented as mean ± SD, N = 4–6 per group. Abbreviations: Ab, antibody; MSC, mesenchymal stem cells; MV, microvesicles.

Article Snippet: If 0 microvesicles derived from normal human lung fibroblasts (NHLF MV, Lonza, Basel, Switzerland) was administered into the perfusate.

Techniques: Derivative Assay

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Microvesicles Derived From Human Mesenchymal Stem Cells Restore Alveolar Fluid Clearance in Human Lungs Rejected for Transplantation

doi: 10.1111/ajt.13271

Figure Lengend Snippet: (A) Compared to T0 and the Control (Perfusion only) at T6, administration of either dose of MSC MV reduced the perfusate pH to more physiological levels. (B) In addition, there was a strong correlation with the change in pH with the AFC, perhaps suggesting that there were increased metabolic activity in the lungs that received MSC MV. Spearman’s rank correlation coefficient, rs = −0.7657. Data are presented as mean ± SD, N = 4–6 per group. *p significant by ANOVA versus T0, †p significant by ANOVA versus Perfusion Group at the corresponding time point. Abbreviations: MSC, mesenchymal stem cells; MV, microvesicles; NHLF, normal human lung fibroblast.

Article Snippet: If 0 microvesicles derived from normal human lung fibroblasts (NHLF MV, Lonza, Basel, Switzerland) was administered into the perfusate.

Techniques: Activity Assay